Journal: Journal of Hard Tissue Biology

Article Title: Effect of circular RNA hsa_circ_0008016 on the Osteogenic and Odontogenic Ability of Stem Cells from Apical Papilla

doi: 10.2485/jhtb.33.105

Figure Lengend Snippet: Figure 5. Effect of hsa-circ-0008016 on OS/OD protein expression of SCAPs. A. Protein expression of BSP, DMP1, DSPP, OCN, RUNX2 in dif- ferent groups. B. Grayscale analysis of A. The expressions of OCN and BSP in the experimental group were significantly increased, while DSPP was significantly decreased. The expressions of RUNX2 and DMP1 were not statistically significant. *P < 0.05, **P < 0.01

Article Snippet: Primary antibodies included BSP, DSPP, DMP1, OCN, RUNX2 (Santa Cruz Biotechnology, USA), and beta-actin (Proteintech Group).

Techniques: Expressing

Journal: Journal of Hard Tissue Biology

Article Title: Effect of circular RNA hsa_circ_0008016 on the Osteogenic and Odontogenic Ability of Stem Cells from Apical Papilla

doi: 10.2485/jhtb.33.105

Figure Lengend Snippet: Figure 4. Effect of hsa-circ-0008016 on OS/OD gene expression of SCAPs. A. The transfection efficacy of pLenti-CMV-Has_circ_0008016- GFP-Puro. The qRT-PCR results showed that the relative expression of hsa_circ_0008016 in cells transfected with overexpression vector was significantly higher than that in the control group. B. qRT-PCR showed that the expressions of DMP1 and BSP were significantly increased in the experimental group, while the expressions of COL1 and DSPP were significantly decreased. The expressions of FGFR1, RUNX2, OCN and ALP were not statistically significant. *P < 0.05, **P < 0.01, and ***P < 0.001

Article Snippet: Primary antibodies included BSP, DSPP, DMP1, OCN, RUNX2 (Santa Cruz Biotechnology, USA), and beta-actin (Proteintech Group).

Techniques: Gene Expression, Transfection, Quantitative RT-PCR, Expressing, Over Expression, Plasmid Preparation, Control

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Rapamycin Reduces Mineral Density and Promotes Beneficial Vascular Remodeling in a Murine Model of Severe Medial Arterial Calcification

doi: 10.1152/ajpheart.00530.2024

Figure Lengend Snippet: A. Graphic illustration of vehicle (DMSO) or 5 mg/kg rapamycin treatment for 25 days in Mgp +/+ and Mgp −/− mice (Created in BioRender. Behzadi, P. (2024) https://BioRender.com/r43g122 ). B. Representative microCT measurement of axial and cross sections of Mgp −/− mice aortas treated with vehicle or 5 mg/kg rapamycin, administered either once or three times a week for 25 days. Scale bar 0.2 mm. n=4 Mgp −/− 1x rapamycin, n=10 Mgp −/− 3x rapamycin, n=12 Mgp −/− vehicle C. Representative Von Kossa staining images of Mgp +/+ and Mgp −/− mice aortas treated with vehicle or rapamycin three times a week for 25 days. Scale bar 0.2 mm. n=10 Mgp −/− rapamycin, n=10 Mgp −/− vehicle, n=10 Mgp +/+ rapamycin, n=10 Mgp +/+ vehicle. D. Representative immunofluorescent images of Runx2 on the Mgp +/+ and Mgp −/− mice aortas treated with vehicle or rapamycin three times a week for 25 days. Scale bar 50 μm. n=10 Mgp −/− rapamycin, n=9 Mgp −/− vehicle, n=10 Mgp +/+ rapamycin, n=10 Mgp +/+ vehicle. Data are shown as means ± SD. P values were calculated using Kruskal-Wallis test (Figure B) and Two-way ANOVA test (Figures C, D).

Article Snippet: Tissues were incubated overnight at 4°C with antibodies for α-SMA (Abcam, ab5694, RRID: AB_2223021, 1:50), Ki67 (Abcam, ab15580, RRID: AB_443209, 1:250), LC3 (Abcam, ab192890, RRID: AB_2827794, 1:50), Mgp (Proteintech, 10734-I-AP, RRID: AB_2297660, 1:25), Myh11 (Invitrogen, MA5–4284, RRID: AB_2911986, 1:50), Runx2 (ABclonal, A2851, RRID: AB_2764676 1:100).

Techniques: Staining

Journal: Cell reports

Article Title: Mapping Distinct Bone Marrow Niche Populations and Their Differentiation Paths

doi: 10.1016/j.celrep.2019.06.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: shRNA-TF510502 (Runx2) , Origene , 4 unique shRNA.

Techniques: Recombinant, shRNA, Construct, Plasmid Preparation, Sequencing, Software

Journal: Journal of tissue engineering

Article Title: Extracellular vesicles derived from human dental pulp stem cells promote osteogenesis of adipose-derived stem cells via the MAPK pathway.

doi: 10.1177/2041731420975569

Figure Lengend Snippet: Figure 1. HDPSCs improved the osteogenic differentiation of hADSCs, probably via EVs. HDPSCs and hADSCs were co-cultured in the presence of GW4869 or DMSO. (a) ALP staining of the co-cultured hDPSCs and hADSCs. (b) Quantitative analysis of ALP activity in the co-cultured hDPSCs and hADSCs. (c) Alizarin Red S staining of the co-cultured hDPSCs and hADSCs. Calcium nodules are indicated by white arrows. (d, e) PCR detection of BMP2 and RUNX2 expression involved in osteogenic differentiation of co-cultured hDPSCs and hADSCs. Data are presented as the mean ± standard deviation of the mean (n = 3), * represents p < 0.05, ** represents p < 0.01.

Article Snippet: Exosome-mediated stem cell differentiation in vitro To evaluate the effect of hDPSC-EVs on osteogenic differentiation of hADSCs, we performed ALP staining, ALP activity assays, qRT-PCR and immunofluorescence assays (IFAs) of RUNX2 and osteocalcin (OCN), as previously described.26,27 The following four groups were assessed: (1) hADSCs cultured in osteoblast-inducing medium (OM) (Cyagen, Santa Clara, CA, USA) alone (0 μg/mL); (2) hADSCs cultured in OM supplemented with 10 μg/mL hDPSC-EVs (10 μg/mL); (3) hADSCs cultured in OM supplemented with 20 μg/mL hDPSC-EVs (20 μg/mL); and (4) hADSCs cultured in OM supplemented with 40 μg/mL hDPSC-EVs (40 μg/mL).

Techniques: Cell Culture, Staining, Activity Assay, Expressing, Standard Deviation

Journal: Journal of tissue engineering

Article Title: Extracellular vesicles derived from human dental pulp stem cells promote osteogenesis of adipose-derived stem cells via the MAPK pathway.

doi: 10.1177/2041731420975569

Figure Lengend Snippet: Figure 3. The effects of hDPSC-EVs on the migration and osteogenic differentiation of hADSCs. (a) Transwell migration assay to detect the effect of hDPSC-EVs on the migration of hADSCs. (b) Quantitative analysis of the effects of hDPSC-EVs on the migration of hADSCs. (c) ALP staining to detect the effect of hDPSC-EVs on osteogenic differentiation of hADSCs. (d) Quantitative detection of ALP activity to detect the effects of hDPSC-EVs on osteogenic differentiation of hADSCs. (e, f) The effects of hDPSC- EVs on the expression of the osteogenic-related genes RUNX2 and BMP2 in hADSCs. (g, h) Immunofluorescence detection of RUNX2 and OCN expression in hADSCs after 7 days incubation with 40 µg/mL hDPSC-EVs. Data are presented as the mean ± standard deviation of the mean (n = 3), * represents p < 0.05, ** represents p < 0.01.

Article Snippet: Exosome-mediated stem cell differentiation in vitro To evaluate the effect of hDPSC-EVs on osteogenic differentiation of hADSCs, we performed ALP staining, ALP activity assays, qRT-PCR and immunofluorescence assays (IFAs) of RUNX2 and osteocalcin (OCN), as previously described.26,27 The following four groups were assessed: (1) hADSCs cultured in osteoblast-inducing medium (OM) (Cyagen, Santa Clara, CA, USA) alone (0 μg/mL); (2) hADSCs cultured in OM supplemented with 10 μg/mL hDPSC-EVs (10 μg/mL); (3) hADSCs cultured in OM supplemented with 20 μg/mL hDPSC-EVs (20 μg/mL); and (4) hADSCs cultured in OM supplemented with 40 μg/mL hDPSC-EVs (40 μg/mL).

Techniques: Migration, Transwell Migration Assay, Staining, Activity Assay, Expressing, Immunofluorescence, Incubation, Standard Deviation

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a ) Immunofluorescence images of Runx2 in BMSCs co-cultured with hydrogels during osteogenic differentiation. ( b ) Number of Runx2-positive cells in BMSCs co-cultured with hydrogels. *** p < 0.005; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques: Immunofluorescence, Cell Culture

Journal: Bioengineering

Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering

doi: 10.3390/bioengineering13020139

Figure Lengend Snippet: ( a – d ) qPCR analysis of OCN , COL-1 , Runx2 and Osterix in BMSCs on Day 7 and 14. * p < 0.05; **** p < 0.0001.

Article Snippet: The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011).

Techniques: